In vitro reconstitution of small GTPase regulation.

Small GTPases play essential roles in the organization of eukaryotic cells. In recent years, it has become clear that their intracellular functions result from intricate biochemical networks of the GTPase and their regulators that dynamically bind to a membrane surface. Due to the inherent complexities of their interactions, however, revealing the underlying mechanisms of action is often difficult to achieve from in vivo studies. This review summarizes in vitro reconstitution approaches developed to obtain a better mechanistic understanding of how small GTPase activities are regulated in space and time. © 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

Citation

Martin Loose, Albert Auer, Gabriel Brognara, Hanifatul Rahmah Budiman, Lukasz Kowalski, Ivana Matijević. In vitro reconstitution of small GTPase regulation. FEBS letters. 2023 Mar;597(6):762-777

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PMID: 36448231

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