Developing an ultrasensitive immunochromatographic assay for authentication of an emergent fraud aminopyrine in herbal tea.

Yalin Fang, Zian Wang, Qiqi Quan, Zhaodong Li, Kangliang Pan, Yi Lei, Xiaojun Yao, Xiangmei Li, Xing Shen, Anastasios Koidis, Hongtao Lei

Food chemistry 2023 Apr 16

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Aminopyrine is a nonsteroidal anti-inflammatory drug only for medical purposes, however, it has been illegally added in traditional Chinese herbal teas for fraud activity recently. In this study, a specific antibody against aminopyrine with IC50 of 3.00 ng/mL was obtained for the first time by a rational hapten design. Furthermore, an ultrasensitive gold nanoparticles immunochromatographic assay (AuNPs-ICA) for determination of aminopyrine based on a portable reader was firstly developed, with cut-off value of 100.00 ng/mL, limit of detection (LOD) of 4.80 ng/mL and limit of quantification (LOQ) of 5.71 ng/mL for herbal tea, respectively. The recovery rates ranged from 93.21 % to 105.61 %, with inter-assay coefficient of variation (CV) from 1.08 % to 3.82 %. Additionally, 24 blind samples were examined simultaneously by AuNPs-ICA and LC-MS/MS, demonstrating a good consistency for each other. The proposed AuNPs-ICA is an ultrasensitive and reliable tool for on-site surveillance screening of fraud additives in herbal tea. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Yalin Fang, Zian Wang, Qiqi Quan, Zhaodong Li, Kangliang Pan, Yi Lei, Xiaojun Yao, Xiangmei Li, Xing Shen, Anastasios Koidis, Hongtao Lei. Developing an ultrasensitive immunochromatographic assay for authentication of an emergent fraud aminopyrine in herbal tea. Food chemistry. 2023 Apr 16;406:135065