3-Hydroxyflavone - bovine serum albumin complex used as fluorescent probe for the detection of pepsin.

Xuanxun Wang, Lin Zhang, Xinyu Yang, Qianjun Zhang

Analytical biochemistry 2023 Feb 01

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In this study, a method for the determination of pepsin using a 3-hydroxyflavone (3-HF)-bovine serum albumin (BSA) complex as a fluorescent probe was established. Under acidic conditions, 3-HF can react with BSA to emit strong green fluorescence, which involves an excited state intramolecular proton transfer and fluorescence resonance energy transfer. After pepsin is added, the fluorescence of the 3-HF-BSA system is quenched due to the hydrolysis of BSA. The degree of 3-HF-BSA fluorescence quenching has a good linear relationship with the concentration of pepsin in the range of 10-80 μg/mL. The correlation coefficient was R2 = 0.9932 and the detection limit was 2.41 μg/mL. This method has high selectivity and good stability and has been used to determine pepsin in real samples with satisfactory results. Copyright © 2022 Elsevier Inc. All rights reserved.

Citation

Xuanxun Wang, Lin Zhang, Xinyu Yang, Qianjun Zhang. 3-Hydroxyflavone - bovine serum albumin complex used as fluorescent probe for the detection of pepsin. Analytical biochemistry. 2023 Feb 01;662:115003