An efficient capture strategy for the purification of human adenovirus type 5 from cell lysates.

Sandra Dietl, Franziska Kiefer, Stefanie Binder, Paul Walther, Harald Sobek, Boris Mizaikoff

Journal of biotechnology 2023 Jan 10

filter terms:

An efficient capture step for human adenovirus type 5 from cell lysate was developed as an initial virus purification step from cell debris supernatant. Organosilane-based polymer particles were synthesized and experimental monomer screening allowed the selection of appropriate functionalities for the development of particles for virus binding. After elution, virus recoveries of 83 % were obtained with significant reduction of matrix proteins and residual host cell DNA. Therefore, the implemented capture strategy for adenovirus via polymer particles provides a scalable and reproducible approach to reduce time and cost during virus purification processes. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Sandra Dietl, Franziska Kiefer, Stefanie Binder, Paul Walther, Harald Sobek, Boris Mizaikoff. An efficient capture strategy for the purification of human adenovirus type 5 from cell lysates. Journal of biotechnology. 2023 Jan 10;361:49-56