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Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells.
Methods in molecular biology (Clifton, N.J.) 2023Sizes of these terms reflect their relevance to your search.
The protozoan parasite, Trypanosoma brucei, offers a simple system to study the growth and duplication of the Golgi. Cell lines stably expressing a photoactivatable GFP attached to an endogenous Golgi protein are permeabilized using digitonin. Photoactivation followed by imaging can then be used to follow the formation of the new Golgi. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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Graham Warren. Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells. Methods in molecular biology (Clifton, N.J.). 2023;2557:53-60
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PMID: 36512209
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