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The protozoan parasite, Trypanosoma brucei, offers a simple system to study the growth and duplication of the Golgi. Cell lines stably expressing a photoactivatable GFP attached to an endogenous Golgi protein are permeabilized using digitonin. Photoactivation followed by imaging can then be used to follow the formation of the new Golgi. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


Graham Warren. Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells. Methods in molecular biology (Clifton, N.J.). 2023;2557:53-60

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PMID: 36512209

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