Studying Golgi Structure and Function by Thin Section TEM.

Here, we describe protocols for chemical fixation and flat embedding to study the Golgi structure by thin section transmission electron microscopy (TEM) and for 3,3'-diaminobenzidine (DAB) cytochemical staining and pre-embedding immunolabelling to localize specific Golgi proteins. Furthermore, we demonstrate how the Golgi morphology can be elucidated by classifying the Golgi membranes using Microscopy Image Browser-a software that provides anonymization, modelling, and annotation. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Helena Vihinen, Eija Jokitalo. Studying Golgi Structure and Function by Thin Section TEM. Methods in molecular biology (Clifton, N.J.). 2023;2557:141-159

Mesh Tags

PMID: 36512215

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