Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System.

Farhana Taher Sumya, Irina D Pokrovskaya, Vladimir V Lupashin

Methods in molecular biology (Clifton, N.J.) 2023

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Conserved oligomeric Golgi (COG) complex orchestrates intra-Golgi retrograde trafficking and glycosylation of macromolecules, but the detailed mechanism of COG action is unknown. Previous studies employed prolonged protein knockout and knockdown approaches which may potentially generate off-target and indirect mutant phenotypes. To achieve a fast depletion of COG subunits in human cells, the auxin-inducible degradation system was employed. This method of protein regulation allows a very fast and efficient depletion of COG subunits, which provides the ability to accumulate COG complex dependent (CCD) vesicles and investigate initial cellular defects associated with the acute depletion of COG complex subunits. This protocol is applicable to other vesicle tethering complexes and can be utilized to investigate anterograde and retrograde intracellular membrane trafficking pathways. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Farhana Taher Sumya, Irina D Pokrovskaya, Vladimir V Lupashin. Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System. Methods in molecular biology (Clifton, N.J.). 2023;2557:365-390