Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer.

Nura Lutfi, Carlos Martínez, José Yélamos

Methods in molecular biology (Clifton, N.J.) 2023

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Poly-ADP-ribosylation of proteins, mediated by the two ADP-ribosyltransferases PARP1 and PARP2 in response to DNA damage, has emerged as a critical mediator of the DNA damage response (DDR). Accordingly, considering the critical role of DDR in cancer, PARP inhibitors (PARPi) have become an important class of therapeutics. PARPi have largely been considered for their intrinsic actions to tumor cells per se. However, these compounds also affect the immune response to tumors. It is now an emerging evidence supporting immunomodulatory roles of PARP1 and PARP2 which can facilitate or impede tumor progression. In this chapter, we describe some protocols to study the immunomodulatory functions of PARP1 and PARP2 in mouse tumor models. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Nura Lutfi, Carlos Martínez, José Yélamos. Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer. Methods in molecular biology (Clifton, N.J.). 2023;2609:195-212