Basidium formation of Rhizopogon roseolus with Pinus thunbergii in agar medium.

Ivan Permana Putra, Tadanori Aimi, Norihiro Shimomura

Fungal biology 2022 Nov-Dec

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The edible ectomycorrhizal (ECM) mushroom Rhizopogon roseolus usually develops basidium and basidiospores in the gleba of its basidiomata. Here, we report a novel production of basidia in laboratory cultures of the edible ECM mushroom. The basidium with sterigma was observed on the old mantle structure (> six months) of the ECM between R. roseolus and Pinus thunbergii in a modified Melin-Norkrans medium that was subjected to a temperature shock from 25 °C to 4 °C. The basidia were cylindrical to clavate, with prominent sterigmata and no basidiospores. The absence of basidiospores might indicate partial development of the basidium structure as a response to environmental stress and incomplete life cycle of R. roseolus. In addition, branched cystidia were evident in two or three clavate-to-ovoid cells. This study suggests the possibility of obtaining the primary mycelium of R. roseolus from pure cultures and may be an alternative genetic source for cultivation purposes. Further observations are required to induce basidiosporogenesis of R. roseolus basidia in an agar medium focusing on manipulation of temperature. Copyright © 2022 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

Citation

Ivan Permana Putra, Tadanori Aimi, Norihiro Shimomura. Basidium formation of Rhizopogon roseolus with Pinus thunbergii in agar medium. Fungal biology. 2022 Nov-Dec;126(11-12):746-751