Yuncheng Man, Erdem Kucukal, Shichen Liu, Ran An, Utku Goreke, William J Wulftange, Zoe Sekyonda, Allison Bode, Jane A Little, Deepa Manwani, Evi X Stavrou, Umut A Gurkan
Biosensors & bioelectronics 2023 Feb 15Neutrophil recruitment to the inflamed endothelium is a multistep process and is of utmost importance in the development of the hallmark vaso-occlusive crisis in sickle cell disease (SCD). However, there lacks a standardized, clinically feasible approach for assessing neutrophil recruitment to the inflamed endothelium for individualized risk stratification and therapeutic response prediction in SCD. Here, we describe a microfluidic device functionalized with E-selectin, a critical endothelial receptor for the neutrophil recruitment process, as a strategy to assess neutrophil binding under physiologic flow in normoxia and clinically relevant hypoxia in SCD. We show that hypoxia significantly enhances neutrophil binding to E-selectin and promotes the formation of neutrophil-platelet aggregates. Moreover, we identified two distinct patient populations: a more severe clinical phenotype with elevated lactate dehydrogenase levels and absolute reticulocyte counts but lowered fetal hemoglobin levels associated with constitutively less neutrophil binding to E-selectin. Mechanistically, we demonstrate that the extent of neutrophil activation correlates with membrane L-selectin shedding, resulting in the loss of ligand interaction sites with E-selectin. We also show that inhibition of E-selectin significantly reduces leukocyte recruitment to activated endothelial cells. Our findings add mechanistic insight into neutrophil-endothelial interactions under hypoxia and provide a clinically feasible means for assessing neutrophil binding to E-selectin using clinical whole blood samples, which can help guide therapeutic decisions for SCD patients. Copyright © 2022 Elsevier B.V. All rights reserved.
Yuncheng Man, Erdem Kucukal, Shichen Liu, Ran An, Utku Goreke, William J Wulftange, Zoe Sekyonda, Allison Bode, Jane A Little, Deepa Manwani, Evi X Stavrou, Umut A Gurkan. A microfluidic device for assessment of E-selectin-mediated neutrophil recruitment to inflamed endothelium and prediction of therapeutic response in sickle cell disease. Biosensors & bioelectronics. 2023 Feb 15;222:114921
PMID: 36521205
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