SHIP1 is required for the formation of neutrophil extracellular traps in rheumatoid arthritis.

Zixue Cheng, Nan L Xia, Ziyan Chen, Mei Li, Zhicheng Tang, Haolin Wu, Xinyi Yan, Yue Wang, Yuchun Wang, Xiaojun Tang, Huayong Zhang, Lingyun Sun

International immunopharmacology 2023 Feb

filter terms:

Aberrant neutrophil extracellular traps (NETs) are involved in the pathogenesis of rheumatoid arthritis (RA). However, the specific pathway leading to NET formation in RA is poorly understood. Therefore, therapies targeting NETs are not available in RA. In this study, we demonstrated Src homology 2 domain-containing inositol phosphatase-1 (SHIP1) function as a hub to regulate NETosis through SHIP1/ p38 MAPK/TNF-α pathway both in vitro and ex vivo and inhibiting SHIP1 expression ameliorated RA symptoms in vivo. Neutrophils from RA patients showed enhanced NETosis as well as increased SHIP1, p38 mitogen-activated protein kinase (MAPK) family expression and tumor necrosis factor-α (TNF-α) expression. Inhibiting SHIP1 in neutrophils using small molecules counteracted the above-mentioned dysregulations and resulted in decrease in NETosis, p38 expression and TNF-α concentration. Consistent with this, SHIP1 agonist led to upregulated p38MAPK and NET formation. Moreover, inhibiting SHIP1 in vivo led to decreased NETosis and showed beneficial therapeutic effects in Collagen-induced arthritis (CIA) mice. Taken together, these results indicated that activation of SHIP1/MAPK/TNF-α pathway was necessary for upregulated NETosis in RA, which provided evidence for targeting SHIP1 in RA treatment. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Zixue Cheng, Nan L Xia, Ziyan Chen, Mei Li, Zhicheng Tang, Haolin Wu, Xinyi Yan, Yue Wang, Yuchun Wang, Xiaojun Tang, Huayong Zhang, Lingyun Sun. SHIP1 is required for the formation of neutrophil extracellular traps in rheumatoid arthritis. International immunopharmacology. 2023 Feb;115:109625