Structural Analyses of the Glycolipids in Lipid Rafts.

Yusuke Suzuki, Kazuya Kabayama

Methods in molecular biology (Clifton, N.J.) 2023

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Lipid rafts are usually isolated from cells or tissues using sucrose gradient ultracentrifugation in the presence of detergents such as Triton X-100 at 4 °C. Although detergents should be removed for further structural characterization following fractionation, these compounds are often difficult to completely remove, especially from the glycolipids. In this chapter, we describe a novel method for the fast and convenient removal of detergents from lipid raft glycolipids following fraction and describe the application of this method. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Yusuke Suzuki, Kazuya Kabayama. Structural Analyses of the Glycolipids in Lipid Rafts. Methods in molecular biology (Clifton, N.J.). 2023;2613:145-152