Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor.

Seu-Na Lee, Hong-Su Jang, Jae-Sung Woo

Methods in molecular biology (Clifton, N.J.) 2023

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CRISPR-cas9-guided adenine base editors (ABEs) site-specifically convert the A-T base pair to G-C base pair in genomic DNA. The intracellular delivery of ABE proteins preassembled with guide RNAs (gRNAs) has shown greatly reduced off-target effects compared with that of plasmids or viral vectors containing ABE and gRNA-encoding sequences. For efficient gene editing by the ribonucleoprotein delivery method, the ABE-gRNA complexes need to be prepared in high purity and quantity. Here we describe the expression and purification procedure of ABEmax, one of high-efficiency ABE versions. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Seu-Na Lee, Hong-Su Jang, Jae-Sung Woo. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor. Methods in molecular biology (Clifton, N.J.). 2023;2606:123-133