Upregulation of RAB7 is related to neuronal pyroptosis after spinal cord injury in rats.

Chao Liu, Qianliang Wang, Shenye Yuan, Sunao Li, Xueshi Chen, Xinqi Huang, Jun Yan, Haiyan Shan, Mingyang Zhang

Journal of chemical neuroanatomy 2023 Mar

filter terms:

Rab7 belongs to the Ras small GTPase superfamily, and abnormal expression of Rab7 can cause neuropathy and lipid metabolism diseases. Studies have shown that Rab7 plays a crucial role in the inner membrane translocase. However, the role of Rab7 in the regulatory mechanisms of cell survival in spinal cord injury remains unknown. We used a rat spinal cord injury (SCI) model to explore the cellular localization and expression of Rab7 after SCI in this study. Western blot analysis showed that Rab7 was expressed in the spinal cord tissue. On the first day, it significantly increased and peaked after SCI on the third day. Furthermore, western blotting also demonstrated that pyroptosis-related protein Gasdermin D (GSDMD), Caspase-1, apoptosis-associated speck-like protein (ASC) expression peaked after the third-day post-injury. Importantly, the immunohistochemistry analysis revealed that Rab7 was completely colocalized with ASC in neurons after SCI. These results suggested that Rab7 was colocalized with NeuN and ASC, involved in the pyroptosis of neurons, and closely related to the spinal cord after injury. Copyright © 2023 Elsevier B.V. All rights reserved.

Citation

Chao Liu, Qianliang Wang, Shenye Yuan, Sunao Li, Xueshi Chen, Xinqi Huang, Jun Yan, Haiyan Shan, Mingyang Zhang. Upregulation of RAB7 is related to neuronal pyroptosis after spinal cord injury in rats. Journal of chemical neuroanatomy. 2023 Mar;128:102229