Protocol for direct measurement of stability and activity of mitochondria electron transport chain complex II.

Himangshu S Bose, Nicole E Doetch

STAR protocols 2023 Mar 17

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Mitochondria electron transport chain (ETC) complex II is essential for steroid metabolism. Here, we present a protocol to measure the stability and activity of mitochondria ETC complex II. We first describe mitochondria isolation from cell lines and tissues. We then detail how to determine the stability of ETC complex II using isothermal calorimetry and quantification of steroidogenesis using activity assays in parallel. Finally, we describe the steps to perform radioimmunoassay (RIA) to confirm the activity of ETC complex II. For complete details on the use and execution of this protocol, please refer to Bose et al. (2020).1. Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Himangshu S Bose, Nicole E Doetch. Protocol for direct measurement of stability and activity of mitochondria electron transport chain complex II. STAR protocols. 2023 Mar 17;4(1):101996