A protocol for collection and infusion of cerebrospinal fluid in mice.

Here, we provide a step-by-step protocol for the collection and intracerebroventricular infusion of cerebrospinal fluid (CSF) in mice. We describe steps to withdraw CSF quickly and abundantly while avoiding blood contamination. Using the Lynch coil technique, we gain functional insights into the collected CSF by slowly infusing minimal amounts of CSF directly to the lateral ventricles of aged mice. This protocol is versatile and can be used to infuse drugs, antibodies, or scarce biological compounds. For complete details on the use and execution of this protocol, please refer to Iram et al. (2022).1. Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Achint Kaur, Steven Shuken, Andrew Chris Yang, Tal Iram. A protocol for collection and infusion of cerebrospinal fluid in mice. STAR protocols. 2023 Mar 17;4(1):102015

Mesh Tags

Substances

PMID: 36638015

search → result