Cytogenetic effects of β-particles in Allium cepa cells used as a biological indicator for radiation damages.

Journal of environmental radioactivity 2023 Apr

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Analysis of cytogenetics effects of ionizing radiation for flora and fauna is essential to determine the impact on these communities and may produce an efficient warning system to avoid harm to human health. Onion (Allium cepa) is a well-established in vivo standard model, and it is widely used in cytogenetics studies for different environmental pollutants. In this work, onion roots were exposed to 0.04-1.44 Gy of β-particles from a 90Sr/90Y source. We investigated the capacity of brief external exposures to β-particles on inducing cytogenetic damages in root meristematic cells of onion aiming to verify if onion can be used as a radiation-sensitive cytogenetic bioindicator. A nonlinear increase in the frequencies of chromosomal aberrations and cells with micronuclei was observed. Onion roots exposed to doses 0.13 Gy or higher of β-particles showed a significant difference (p<0.05) in these frequencies when compared to the unirradiated group. The frequencies of these endpoints showed to be suitable to assess the difference in the dose of beta radiation received from 0.36 Gy. Our research shows the potential of using cytogenetic effects in Allium cepa cells as a biological indicator for a first screening of genotoxic damages induced by brief external exposures to β-particles. Copyright © 2023 Elsevier Ltd. All rights reserved.

Citation

Magno Nogueira Xavier, July Anne Torres Novaes, Alef Cyntille Cavalcante Silva, Anderson Vinicius Silva Alves, Maria José Bryanne Araujo Santos, Silmara de Moraes Pantaleão, Ricardo Scher, Francesco d'Errico, Susana Oliveira de Souza. Cytogenetic effects of β-particles in Allium cepa cells used as a biological indicator for radiation damages. Journal of environmental radioactivity. 2023 Apr;259-260:107109