Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry.

Glenda Vasku, Niyazi Acar, Olivier Berdeaux

Methods in molecular biology (Clifton, N.J.) 2023

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Analysis and quantification of ether-lipid phospholipid species-also known as plasmalogens-is a crucial step in the study of the biological functions played by these lipids. Application of analytical separation methods and high-resolution mass spectrometry has gained much attention in this regard, while resolution issues and time-consuming sequences interfered with these advances. Herein, we describe a simple and rapid method for the analysis of plasmalogen (Pl) species by HILIC-HRMS. This method is able to identify and quantify relative levels of ethanolamine-plasmalogens (PlsEtn) and choline-plasmalogens (PlsCho) in biological matrices such as whole blood, plasma, erythrocytes, and also retina. Moreover, we provide a detailed and modified lipid extraction method that is applicable to almost all biological matrices. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Glenda Vasku, Niyazi Acar, Olivier Berdeaux. Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry. Methods in molecular biology (Clifton, N.J.). 2023;2625:259-267