In Vitro Investigation of Hyaluronan/CD44 Network.

Theodore Karalis, Paraskevi Heldin, Spyros S Skandalis

Methods in molecular biology (Clifton, N.J.) 2023

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Hyaluronan is one of the most influential components of the extracellular matrix. It is involved in the regulation of normal tissue function and architecture, while its metabolism is perturbed in a multitude of human diseases like inflammation, cancer, and viral infection. Given the implication of hyaluronan in a vast array of diseases, we describe here assays that can be utilized to study the quantity, size, subcellular localization, and binding capacity of hyaluronan by cells as well as its interactions with its major cellular receptor, CD44. Hopefully, these protocols will provide researchers with useful tools to study the complex hyaluronan biology. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Theodore Karalis, Paraskevi Heldin, Spyros S Skandalis. In Vitro Investigation of Hyaluronan/CD44 Network. Methods in molecular biology (Clifton, N.J.). 2023;2619:169-186