Correlation Engine 2.0
Clear Search sequence regions

  • apoptosis (1)
  • bag (4)
  • camptotheca (1)
  • camptothecin (11)
  • cell cycle (3)
  • cyclin (8)
  • dna damage (1)
  • drosophila (2)
  • drosophila proteins (2)
  • g1 phase (1)
  • germ cells (2)
  • GSCs (1)
  • marbles (4)
  • phase (1)
  • regulates (1)
  • stem cell (2)
  • top1 (1)
  • tree (1)
  • Sizes of these terms reflect their relevance to your search.

    Camptothecin (CPT), first isolated from Chinese tree Camptotheca acuminate, produces rapid and prolonged inhibition of DNA synthesis and induction of DNA damage by targeting topoisomerase I (top1), which is highly activated in cancer cells. CPT thus exhibits remarkable anticancer activities in various cancer types, and is a promising therapeutic agent for the treatment of cancers. However, it remains to be uncovered underlying its cytotoxicity toward germ cells. In this study we found that CPT, a cell cycle-specific anticancer agent, reduced fecundity and exhibited significant cytotoxicity toward GSCs and two-cell cysts. We showed that CPT induced GSC loss and retarded two-cell cysts differentiation in a niche- or apoptosis-independent manner. Instead, CPT induced ectopic expression of a differentiation factor, bag of marbles (Bam), and regulated the expression of cyclin A, which contributed to GSC loss. In addition, CPT compromised two-cell cysts differentiation by decreasing the expression of Bam and inducing cell arrest at G1/S phase via cyclin A, eventually resulting in two-cell accumulation. Collectively, this study demonstrates, for the first time in vivo, that the Bam-cyclin A axis is involved in CPT-mediated germline stem cell loss and two-cell cysts differentiation defects via inducing cell cycle arrest, which could provide information underlying toxicological effects of CPT in the productive system, and feature its potential to develop as a pharmacology-based germline stem cell regulation agent.


    Jing Zhang, Shijie Zhang, Zhipeng Sun, Yu Cai, Guohua Zhong, Xin Yi. Camptothecin Effectively Regulates Germline Differentiation through Bam-Cyclin A Axis in Drosophila melanogaster. International journal of molecular sciences. 2023 Jan 13;24(2)

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 36675143

    View Full Text