A novel fluorescent endoplasmic reticulum marker for super-resolution imaging in live cells.

Kai Han, Shuhan Huang, Jie Kong, Yang Yang, Lei Shi, Yali Ci

FEBS letters 2023 Mar

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Endoplasmic reticulum (ER) is a highly complicated and dynamic organelle that actively changes its shape and communicates with other organelles. Visualization of ER in live cells is of great importance to understand cellular activities. Here, we designed a novel ER marker, RR-mNeonGreen, which comprised an N-terminal ER retention signal, a bright fluorescent protein (mNeonGreen), and a C-terminal transmembrane region. Colocalization of RR-mNeonGreen with mCherry-KDEL verified that RR-mNeonGreen perfectly labeled the ER. RR-mNeonGreen showed better continuity of ER tubules when imaged by super-resolution microscopy. Moreover, RR-mNeonGreen is competent for live-cell imaging of ER dynamics and tracing of the interaction between ER and mitochondria at high spatiotemporal resolution. In summary, RR-mNeonGreen is a novel ER marker for super-resolution live-cell imaging with multiple merits. © 2023 Federation of European Biochemical Societies.

Citation

Kai Han, Shuhan Huang, Jie Kong, Yang Yang, Lei Shi, Yali Ci. A novel fluorescent endoplasmic reticulum marker for super-resolution imaging in live cells. FEBS letters. 2023 Mar;597(5):693-701