Direct cell extraction of membrane proteins for structure-function analysis.

Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM. © 2023. The Author(s).

Citation

Ieva Drulyte, Aspen Rene Gutgsell, Pilar Lloris-Garcerá, Michael Liss, Stefan Geschwindner, Mazdak Radjainia, Jens Frauenfeld, Robin Löving. Direct cell extraction of membrane proteins for structure-function analysis. Scientific reports. 2023 Jan 25;13(1):1420

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PMID: 36697499

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