Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary.

Ildikó Kristó, Péter Borkúti, Zoltán Kovács, Anikó Szabó, Szilárd Szikora, Péter Vilmos

Methods in molecular biology (Clifton, N.J.) 2023

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Much evidence supports the presence of cytoskeletal elements in the nucleus; however, the exact functions of these proteins in the nucleus are still uncertain. Of the cytoskeletal proteins, the activity and biological significance of nuclear actin has been the most extensively researched. It is now clear that actin performs essential tasks both in the cytoplasm and the nucleus, and that the dynamic balance between the large cytoplasmic and the significantly smaller nuclear actin pools is maintained by robust transport mechanisms. Therefore, the compartment-specific manipulation or investigation of actin has been an enormous challenge. Here, we present a protocol for the detection of actin in isolated nuclear protein fractions from Drosophila ovaries. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Ildikó Kristó, Péter Borkúti, Zoltán Kovács, Anikó Szabó, Szilárd Szikora, Péter Vilmos. Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary. Methods in molecular biology (Clifton, N.J.). 2023;2626:353-364