FACS to Identify Immune Subsets in Mouse Brain and Spleen.

Mary K Malone, Thomas A Ujas, Katherine M Cotter, Daimen R S Britsch, Jenny Lutshumba, Jadwiga Turchan-Cholewo, Ann M Stowe

Methods in molecular biology (Clifton, N.J.) 2023

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Flow cytometry enables the multi-parametric quantification of cell types, especially in immunophenotyping of unique immune cell subsets that can either contribute to or ameliorate pathology. For tissues to be used in such analyses, single-cell suspensions must be created. Here we describe protocols for preparing single-cell suspensions of mouse spleen and brain tissue, as well as the steps for fluorescently activated cell staining/sorting (FACS). Specifically, this protocol enables the isolation of lymphocytes for the study of immune responses during various diseases, such as long-term neuroinflammation following ischemic stroke. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Mary K Malone, Thomas A Ujas, Katherine M Cotter, Daimen R S Britsch, Jenny Lutshumba, Jadwiga Turchan-Cholewo, Ann M Stowe. FACS to Identify Immune Subsets in Mouse Brain and Spleen. Methods in molecular biology (Clifton, N.J.). 2023;2616:213-229