Yibin Wang, Jiaxin Yu, Mengwei Jiang, Wenlong Lei, Xingtan Zhang, Haibao Tang
Methods in molecular biology (Clifton, N.J.) 2023Polyploidy has been observed throughout major eukaryotic clades and has played a vital role in the evolution of angiosperms. Recent polyploidizations often result in highly complex genome structures, posing challenges to genome assembly and phasing. Recent advances in sequencing technologies and genome assembly algorithms have enabled high-quality, near-complete chromosome-level assemblies of polyploid genomes. Advances in novel sequencing technologies include highly accurate single-molecule sequencing with HiFi reads, chromosome conformation capture with Hi-C technique, and linked reads sequencing. Additionally, new computational approaches have also significantly improved the precision and reliability of polyploid genome assembly and phasing, such as HiCanu, hifiasm, ALLHiC, and PolyGembler. Herein, we review recently published polyploid genomes and compare the various sequencing, assembly, and phasing approaches that are utilized in these genome studies. Finally, we anticipate that accurate and telomere-to-telomere chromosome-level assembly of polyploid genomes could ultimately become a routine procedure in the near future. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Yibin Wang, Jiaxin Yu, Mengwei Jiang, Wenlong Lei, Xingtan Zhang, Haibao Tang. Sequencing and Assembly of Polyploid Genomes. Methods in molecular biology (Clifton, N.J.). 2023;2545:429-458
PMID: 36720827
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