Establishment of matrix metalloproteinase 3 time-resolved immunoassay and some potential clinical applications.

To develop a highly sensitive time-resolved fluorescence immunoassay (TRFIA) for the detection of serum matrix metalloproteinase-3 (MMP-3) and to assess MMP-3's clinical value in patients with colorectal cancer (CRC).st. MMP-3 levels were established using the double antibody sandwich technique. The MMP-3 TRFIA technique was developed and optimized, and its linearity, sensitivity, accuracy, specificity, and recovery were assessed. Then, serum concentrations in healthy individuals and patients with CRC were determined by MMP-3 TRFIA. The linear range of MMP-3 TRFIA was 0.73-500 ng/mL. MMP-3 TRFIA had an intra-batch precision range of 2.16%-7.10% percent and an inter-batch precision range of 3.99%-11.21%. MMP-3, tumor-associated trypsinogen 2, and AFP had no cross reaction.The recovery is between 90% and 110%, and had no serum interference. Patients with CRC had serum MMP-3 levels (73.95 ± 78.43 ng/mL) that were considerably higher than those of healthy individuals (21.45 ± 11.12 ng/mL), and those with metastasis had serum MMP-3 levels (95.89 ± 76.21 ng/mL) that were considerably higher than those of patients without metastasis (52.74 ± 47.25 ng/mL). A highly sensitive MMP-3 TRFIA assay was successfully developed, and serum MMP-3 may be associated with CRC invasion and metastasis. Therefore, MMP-3 can be used in the auxiliary diagnosis of CRC. Copyright © 2023 Elsevier Inc. All rights reserved.

Citation

Yulin Fu, Xiaoyan Wang, Xindong Chen, Jianfeng Hong, Yuan Qin, Zixuan Zhou, Xiumei Zhou, Yigang Wang, Jingnan Zhou, Hongming Fang, Pengfei Liu, Biao Huang. Establishment of matrix metalloproteinase 3 time-resolved immunoassay and some potential clinical applications. Analytical biochemistry. 2023 Apr 01;666:115072

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PMID: 36738873

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