The past, present, and future of artificial zinc finger proteins: design strategies and chemical and biological applications.

Zinc finger proteins are abundant in the human proteome and are responsible for a variety of functions. The domains that constitute zinc finger proteins are compact spherical structures, each comprising approximately 30 amino acid residues, but they also have precise molecular factor functions: zinc binding and DNA recognition. Due to the biological importance of zinc finger proteins and their unique structural and functional properties, many artificial zinc finger proteins have been created and are expected to improve their functions and biological applications. In this study, we review previous studies on the redesign and application of artificial zinc finger proteins, focusing on the experimental results obtained by our research group. In addition, we systematically review various design strategies used to construct artificial zinc finger proteins and discuss in detail their potential biological applications, including gene editing. This review will provide relevant information to researchers involved or interested in the field of artificial zinc finger proteins as a potential new treatment for various diseases. © 2023. The Author(s), under exclusive licence to Society for Biological Inorganic Chemistry (SBIC).

Citation

Shigeru Negi, Miki Imanishi, Mami Hamori, Yuka Kawahara-Nakagawa, Wataru Nomura, Kanae Kishi, Nobuhito Shibata, Yukio Sugiura. The past, present, and future of artificial zinc finger proteins: design strategies and chemical and biological applications. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry. 2023 Apr;28(3):249-261

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PMID: 36749405

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