V V Antsiferova, A G Ven'iaminova, M N Repkova, V I Iamkovoĭ
Bioorganicheskaia khimiia 1987 AugOligoribonucleotide ApUpG(pU)6(pA)5pAp (I) has been prepared by means of RNA ligase. ApUpG, synthesised by the phosphotriester approach, was elongated in the 3'-direction by adding (pU)6 and then (pA)6, which was 3'-blocked with the phosphate or with the periodate-oxidized AMP residue, the latter giving considerably lower level of by-products. Condensation of ApUpG(pU)6 with blocked (pA)6 was carried out under conditions optimal for poor acceptors (20 degrees C, 48 h, pH 8,7) to afford (I) with the yield of 20% (105 OE260); ApUpG(pU)6(pA)10pAp was identified as a byproduct.
V V Antsiferova, A G Ven'iaminova, M N Repkova, V I Iamkovoĭ. RNA-ligase of bacteriophage T4. IV. Synthesis of pentadecaribonucleotide ApUpG(pU)6(pA)5pAp]. Bioorganicheskaia khimiia. 1987 Aug;13(8):1031-6
PMID: 3675646
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