Molecular characterization of horse nettle virus A, a new member of subgroup B of the genus Nepovirus.

A new positive-strand RNA virus was discovered in a horse nettle plant, using high-throughput sequencing (HTS), and its complete genome, consisting of RNA1 and RNA2, which are 7522 and 4710 nucleotides in length, respectively, was characterized. Each genome segment contains a single open reading frame flanked by 5' and 3' untranslated regions (UTRs), followed by a poly(A) tail at the 3' end. The encoded proteins have the highest amino acid sequence identity (55% and 45%) to the polyprotein encoded by RNA1 of tomato black ring virus (TBRV) and RNA2 of potato virus B (PVB), respectively. Its genome organization and phylogenetic relationship to other nepoviruses suggested that this virus is a novel member of subgroup B, and recombination analysis revealed its evolutionary history within the subgroup. These results suggest the new virus, provisionally named "horse nettle virus A", represents a new species within the genus Nepovirus. © 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.

Citation

Jing Zhou, Xiaojun Hu, Paulo Vieira, Benjamin Atha, Clint McFarland, Joseph A Foster, Oscar P Hurtado-Gonzales. Molecular characterization of horse nettle virus A, a new member of subgroup B of the genus Nepovirus. Archives of virology. 2023 Feb 11;168(3):86

Mesh Tags

Substances

PMID: 36773166

search → result