Structural insights into the evolution of late steps of translation initiation in the three domains of life.

In eukaryotes and in archaea late steps of translation initiation involve the two initiation factors e/aIF5B and e/aIF1A. These two factors are also orthologous to the bacterial IF2 and IF1 proteins, respectively. Recent cryo-EM studies showed how e/aIF5B and e/aIF1A cooperate on the small ribosomal subunit to favor the binding of the large ribosomal subunit and the formation of a ribosome competent for elongation. In this review, pioneering studies and recent biochemical and structural results providing new insights into the role of a/eIF5B in archaea and eukaryotes will be presented. Recent structures will also be compared to orthologous bacterial initiation complexes to highlight domain-specific features and the evolution of initiation mechanisms. Copyright © 2023 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

Citation

Ramy Kazan, Gabrielle Bourgeois, Christine Lazennec-Schurdevin, Pierre-Damien Coureux, Yves Mechulam, Emmanuelle Schmitt. Structural insights into the evolution of late steps of translation initiation in the three domains of life. Biochimie. 2024 Feb;217:31-41

Mesh Tags

Substances

PMID: 36773835

search → result