Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology.

Double digest restriction-site associated DNA sequencing (ddRADseq) technology combines genome reduced representation by digestion with two restriction enzymes and next generation sequencing (NGS) to obtain thousands of markers (SNP, SSR, and InDels) and genotype tens to hundreds of samples simultaneously. In this chapter, we describe a 96-plex derived ddRADseq protocol that can be set up to obtain different depth of coverage per locus and can be exploited to model and non-model plant species. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Natalia Cristina Aguirre, Carla Valeria Filippi, Pablo Alfredo Vera, Andrea Fabiana Puebla, Giusi Zaina, Verónica Viviana Lia, Susana Noemí Marcucci Poltri, Norma Beatriz Paniego. Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology. Methods in molecular biology (Clifton, N.J.). 2023;2638:37-57

Mesh Tags

PMID: 36781634

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