Preparation and Characterization of Trastuzumab Fab-Conjugated Liposomes (Immunoliposomes).

Immunoliposomes are made by conjugating antibodies or antibody fragments on liposome surfaces. Antibody fragments Fab', single-chain Fv fragments (scFv), or new constructs such as nanobodies are commonly used instead of whole IgGs for reduced risk immunogenicity. Here we described the preparation and characterization of immunoliposome-containing trastuzumab Fabs on the surface. The targeting ligand Fab-PEG-DSPE was synthesized by site-specific coupling between the C-terminal cysteine of the Fab and the maleimide group at the distal end of a DSPE-PEG. It was incorporated into preformed liposomes at 60 °C above the lipid bilayer phase transition temperature. The binding avidity of the immunoliposomes containing different Fab valencies was characterized using biolayer interferometry. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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ShanShan Jin, Huimin Li, Yuhong Xu. Preparation and Characterization of Trastuzumab Fab-Conjugated Liposomes (Immunoliposomes). Methods in molecular biology (Clifton, N.J.). 2023;2622:197-205

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PMID: 36781762

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