Chondromodulin is necessary for cartilage callus distraction in mice.

Kiminori Yukata, Chisa Shukunami, Yoshito Matsui, Aki Takimoto, Tomohiro Goto, Mitsuhiko Takahashi, Atsushi Mihara, Tetsuya Seto, Takashi Sakai, Yuji Hiraki, Natsuo Yasui

PloS one 2023

filter terms:

Chondromodulin (Cnmd) is a glycoprotein known to stimulate chondrocyte growth. We examined in this study the expression and functional role of Cnmd during distraction osteogenesis that is modulated by mechanical forces. The right tibiae of the mice were separated by osteotomy and subjected to slow progressive distraction using an external fixator. In situ hybridization and immunohistochemical analyses of the lengthened segment revealed that Cnmd mRNA and its protein in wild-type mice were localized in the cartilage callus, which was initially generated in the lag phase and was lengthened gradually during the distraction phase. In Cnmd null (Cnmd-/-) mice, less cartilage callus was observed, and the distraction gap was filled by fibrous tissues. Additionally, radiological and histological investigations demonstrated delayed bone consolidation and remodeling of the lengthened segment in Cnmd-/- mice. Eventually, Cnmd deficiency caused a one-week delay in the peak expression of VEGF, MMP2, and MMP9 genes and the subsequent angiogenesis and osteoclastogenesis. We conclude that Cnmd is necessary for cartilage callus distraction. Copyright: © 2023 Yukata et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation

Kiminori Yukata, Chisa Shukunami, Yoshito Matsui, Aki Takimoto, Tomohiro Goto, Mitsuhiko Takahashi, Atsushi Mihara, Tetsuya Seto, Takashi Sakai, Yuji Hiraki, Natsuo Yasui. Chondromodulin is necessary for cartilage callus distraction in mice. PloS one. 2023;18(2):e0280634