Integrated tandem affinity protein purification using the polyhistidine plus extra 4 amino acids (HiP4) tag system.

Yoko Ino, Yutaro Yamaoka, Kiho Tanaka, Kei Miyakawa, Mayuko Nishi, Yasuyoshi Hatayama, Hirokazu Kimura, Yayoi Kimura, Akihide Ryo

Proteomics 2023 Jun

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Peptide tag systems are a robust biophysical and biochemical method that is widely used for protein detection and purification. Here, we developed a novel tag system termed "HiP4" (histidine plus four amino acids) whose epitope sequence comprises only seven amino acids (HHHDYDI) that partially overlap with the conventional 6x histidine tag (6xHis-tag). We produced a monoclonal antibody against the HiP4 tag that can be used in multiple immunoassays with high specificity and affinity. Using this system, we developed a tandem affinity purification (TAP) and mass spectrometry (TAP-MS) system for comprehensive protein interactome analysis. The integrated use of nickel bead purification followed by HiP4 tag immunoprecipitation made it possible to reduce nonspecific binding and improve selectivity, leading to the recovery of previously unrecognized proteins that interact with hepatitis B virus X (HBx) protein or TAR DNA-binding protein 43 (TARDBP or TDP-43). Our results indicate that this system may be viable as a simple and powerful tool for TAP-MS that can achieve low background and high selectivity in comprehensive protein-protein interaction analyses. © 2023 Wiley-VCH GmbH.

Citation

Yoko Ino, Yutaro Yamaoka, Kiho Tanaka, Kei Miyakawa, Mayuko Nishi, Yasuyoshi Hatayama, Hirokazu Kimura, Yayoi Kimura, Akihide Ryo. Integrated tandem affinity protein purification using the polyhistidine plus extra 4 amino acids (HiP4) tag system. Proteomics. 2023 Jun;23(11):e2200334