Manipulation of Murine Mitochondrial DNA Heteroplasmy with mtZFNs.

Pavel A Nash, Michal Minczuk

Methods in molecular biology (Clifton, N.J.) 2023

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Mouse models of mitochondrial DNA mutations hold promise in the development and optimization of mitochondrial gene therapy technology and for gathering pre-clinical data prior to human trials. Their suitability for this purpose stems from the high similarity of human and murine mitochondrial genomes and the increasing availability of rationally designed AAV vectors capable of selectively transducing murine tissues. Our laboratory routinely optimizes mitochondrially targeted zinc finger nucleases (mtZFNs), the compactness of which makes them highly suitable for downstream AAV-based in vivo mitochondrial gene therapy. This chapter discusses the necessary precautions for the robust and precise genotyping of the murine mitochondrial genome as well as the optimization of mtZFNs intended for subsequent use in vivo. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Pavel A Nash, Michal Minczuk. Manipulation of Murine Mitochondrial DNA Heteroplasmy with mtZFNs. Methods in molecular biology (Clifton, N.J.). 2023;2615:329-344