In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles.

Katsumi Imada, Hiroyuki Terashima

Methods in molecular biology (Clifton, N.J.) 2023

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The flagellar axial proteins are transported across the cytoplasmic membrane into the central channel of the growing flagellum via the flagellar protein export apparatus, a member of the type III secretion system (T3SS). To reveal the molecular mechanism of protein transport by the T3SS, accurate measurement of protein transport under various conditions is essential. In this chapter, we describe an in vitro method for flagellar protein transport assay using inverted membrane vesicles (IMVs) prepared from Salmonella cells. This method can easily and precisely control the condition around the T3SS and be applied to other T3SSs. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Katsumi Imada, Hiroyuki Terashima. In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles. Methods in molecular biology (Clifton, N.J.). 2023;2646:17-26