A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells.

Anna Khabarova, Galina Koksharova, Pavel Salnikov, Polina Belokopytova, Roman Mungalov, Inna Pristyazhnuk, Artem Nurislamov, Maria Gridina, Veniamin Fishman

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2023 Feb 26

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Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations. © 2023. The Author(s), under exclusive licence to Springer Nature B.V.

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Anna Khabarova, Galina Koksharova, Pavel Salnikov, Polina Belokopytova, Roman Mungalov, Inna Pristyazhnuk, Artem Nurislamov, Maria Gridina, Veniamin Fishman. A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology. 2023 Feb 26;31(1):11