Metabolic engineering of Saccharomyces cerevisiae for glycerol utilization.

Due to its wide availability, glycerol is considered as a promising alternative feedstock for microbial fermentation. As a model eukaryote, Saccharomyces cerevisiae is commonly adopted for bioproduction of various bulk and value-added chemicals, but it does not efficiently utilize glycerol. In this review, the metabolic pathway of glycerol and its regulation in S. cerevisiae are first introduced. Then, strategies, including metabolic engineering of the endogenous pathway, introduction of exogenous pathways, adaptive evolution, and reverse metabolic engineering, are summarized for improving the glycerol utilization in S. cerevisiae. Finally, methods for further improving glycerol utilization by S. cerevisiae are proposed. This review provides insights for designing engineered S. cerevisiae for efficient utilization of glycerol. © The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.

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Ziqian Yu, Zhao Chang, Yinhua Lu, Han Xiao. Metabolic engineering of Saccharomyces cerevisiae for glycerol utilization. FEMS yeast research. 2023 Jan 04;23

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PMID: 36869777

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