Liquid crystal-based sensor for real-time detection of paraoxon pesticides based on acetylcholinesterase enzyme inhibition.

A liquid crystal-based assay (LC) was developed to monitor paraoxon by incorporating a Cu2+ -coated substrate and the inhibitory effect of paraoxon with acetylcholinesterase (AChE). We observed that thiocholine (TCh), a hydrolysate of AChE and acetylthiocholine (ATCh), interfered with the alignment of 5CB films through a reaction between Cu2+ ions and the thiol moiety of TCh. The catalytic activity of AChE was inhibited in the presence of paraoxon due to the irreversible interaction between TCh and paraoxon; consequently, no TCh molecule was available to interact with Cu2+ on the surface. This resulted in a homeotropic alignment of the liquid crystal. The proposed sensor platform sensitively quantified paraoxon with a detection limit of 2.20 ± 0.11 (n = 3) nM within a range of 6 to 500 nM. The specificity and reliability of the assay were verified by measuring paraoxon in the presence of various suspected interfering substances and spiked samples. As a result, the sensor based on LC can potentially be used as a screening tool for accurate evaluation of paraoxon and other organophosphorus compounds. © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

Citation

Duong Song Thai Duong, Chang-Hyun Jang. Liquid crystal-based sensor for real-time detection of paraoxon pesticides based on acetylcholinesterase enzyme inhibition. Mikrochimica acta. 2023 Mar 09;190(4):122

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PMID: 36890280

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