SOCS2 regulates alveolar bone loss in Aggregatibacter actinomycetemcomitans-induced periodontal disease.

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2023 Apr

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The role of suppressor of cytokine signaling 2 (SOCS2) in Aggregatibacter actinomycetemcomitans (Aa)-induced alveolar bone loss is unknown; thus, it was investigated in this study. Alveolar bone loss was induced by infecting C57BL/6 wild-type (WT) and Socs2-knockout (Socs2-/-) mice with Aa. Bone parameters, bone loss, bone cell counts, the expression of bone remodeling markers, and cytokine profile were evaluated by microtomography, histology, qPCR, and/or ELISA. Bone marrow cells (BMC) from WT and Socs2-/- mice were differentiated in osteoblasts or osteoclasts for analysis of the expression of specific markers. Socs2-/- mice intrinsically exhibited irregular phenotypes in the maxillary bone and an increased number of osteoclasts. Upon Aa infection, SOCS2 deficiency resulted in the increased alveolar bone loss, despite decreased proinflammatory cytokine production, in comparison to the WT mice. In vitro, SOCS2 deficiency resulted in the increased osteoclasts formation, decreased expression of bone remodeling markers, and proinflammatory cytokines after Aa-LPS stimulus. Collectively, data suggest that SOCS2 is a regulator of Aa-induced alveolar bone loss by controlling the differentiation and activity of bone cells, and proinflammatory cytokines availability in the periodontal microenvironment and an important target for new therapeutic strategies. Thus, it can be helpful in preventing alveolar bone loss in periodontal inflammatory conditions. © 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.

Citation

Mariana Rates Gonzaga Santos, Ian de Meira Chaves, Celso Martins Queiroz-Junior, Allysson Thiago Cramer, Thales Augusto Anestino, Anna Clara Paiva Menezes Dos Santos, Paulo Gaio Leite, Soraia Macari, Breno Rocha Barrioni, Marivalda de Magalhães Pereira, Mauro Martins Teixeira, Danielle da Glória de Souza, Mila Fernandes Moreira Madeira, Fabiana Simão Machado. SOCS2 regulates alveolar bone loss in Aggregatibacter actinomycetemcomitans-induced periodontal disease. Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 2023 Apr;72(4):859-873