Pseudotyped Viruses for Marburgvirus and Ebolavirus.

Li Zhang, Shou Liu, Youchun Wang

Advances in experimental medicine and biology 2023

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Marburg virus (MARV) and Ebola virus (EBOV) of the Filoviridae family are the most lethal viruses in terms of mortality rate. However, the development of antiviral treatment is hampered by the requirement for biosafety level-4 (BSL-4) containment. The establishment of BSL-2 pseudotyped viruses can provide important tools for the study of filoviruses. This chapter summarizes general information on the filoviruses and then focuses on the construction of replication-deficient pseudotyped MARV and EBOV (e.g., lentivirus system and vesicular stomatitis virus system). It also details the potential applications of the pseudotyped viruses, including neutralization antibody detection, the study of infection mechanisms, the evaluation of antibody-dependent enhancement, virus entry inhibitor screening, and glycoprotein mutation analysis. © 2023. The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

Citation

Li Zhang, Shou Liu, Youchun Wang. Pseudotyped Viruses for Marburgvirus and Ebolavirus. Advances in experimental medicine and biology. 2023;1407:105-132