Total chemical synthesis of sumoylated histone H4 reveals negative biochemical crosstalk with histone ubiquitylation.

Sumeet K Singh, Andres Reyna, Xiaowen Xie, Haibin Mao, Meihuan Ji, Ning Zheng, Peter L Hsu, Champak Chatterjee

Chemical communications (Cambridge, England) 2023 Mar 30

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An efficient total chemical synthesis of site-specifically sumoylated histone H4 was undertaken to generate homogenously modified mononucleosomes. These were tested as substrates in biochemical assays with the histone H2B-specific ubiquitin ligases Rad6 and Bre1, which revealed the strong inhibition of H2B ubiquitylation by SUMO. This novel negative biochemical crosstalk between SUMO and ubiquitin was also confirmed to exist in human cells.

Citation

Sumeet K Singh, Andres Reyna, Xiaowen Xie, Haibin Mao, Meihuan Ji, Ning Zheng, Peter L Hsu, Champak Chatterjee. Total chemical synthesis of sumoylated histone H4 reveals negative biochemical crosstalk with histone ubiquitylation. Chemical communications (Cambridge, England). 2023 Mar 30;59(27):4063-4066