Glycosylated quantum dots as fluorometric nanoprobes for trehalase.

Trehalase is an important enzyme in the metabolic cascades of many organisms, catalysing the hydrolysis of the disaccharide trehalose. Herein we describe the first examples of fluorometric nanoprobes for detection of trehalase, based on trehalose-functionalised quantum dots (QDs). QDs cross-linked with trehalose form aggregates, which are released upon enzymatic cleavage of the trehalose glycosidic bond proportionally to the enzyme concentration, offering a unique and efficient approach for specific sensing of this biologically important enzyme.

Citation

Danielle D Barnes, Vera Kuznetsova, Anastasia Visheratina, Finn Purcell-Milton, Mikhail A Baranov, Dylan M Lynch, Harlei Martin, Yurii K Gun'ko, Eoin M Scanlan. Glycosylated quantum dots as fluorometric nanoprobes for trehalase. Organic & biomolecular chemistry. 2023 Apr 05;21(14):2905-2909

Mesh Tags

Substances

PMID: 36942668

search → result