Potential roles of IFI44 genes in high resistance to Vibrio in hybrids of Argopecten scallops.

Xiaoxu Qu, Xia Wang, Bo Liu, Min Chen, Junhao Ning, Haijun Liu, Guilong Liu, Xin Xu, Xiaotong Zhang, Kai Yu, He Xu, Xia Lu, Chunde Wang

Fish & shellfish immunology 2023 Apr

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Vibrio bacteria are often fatal to aquatic organisms and selection of Vibrio-resistant strains is warranted for aquaculture animals. In this study, we found that hybrids between bay scallops and Peruvian scallops exhibited significantly higher resistance to Vibrio challenge, but little is available on its mechanism. Interferon induced protein 44 (IFI44), a member of the type I interferon (IFN) family, plays an important role in the IFN immune response in invertebrates, which may also participate in the resistance to Vibrio in scallops. To explore the roles of IFI44 genes in the resistance to Vibrio, they were identified and characterized in the bay scallop (designated as AiIFI44), the Peruvian scallop (designated as ApIFI44), and their reciprocal hybrids (designated as AipIFI44 and ApiIFI44, respectively). Their open reading frame (ORF) sequences were all 1434 bp, encoding 477 amino acids, but with large variations among the four genes. The AipIFI44 and ApiIFI44 exhibited higher similarity with ApIFI44 than with AiIFI44. All four genes have a TLDc structural domain with significant variations in sequences among them. Predicted differences in conformation and posttranslational modifications may lead to altered protein activity. We further demonstrated that the AiIFI44, AipIFI44 and ApiIFI44 expressed in all the tested tissues, with the highest expression in the gills and hepatopancreas. In response to Vibrio anguillarum challenge, the profile of mRNA expression of IFI44 gene differed among the bay scallops and the two hybrids. In the bay scallops, it increased at 6 h but dramatically decreased after 12-48 h. However, the mRNA expression of both AipIFI44 and ApiIFI44 decreased at 6 h but continuously increased thereafter and reached the highest value at 48 h. The results in the present study suggest the immune responds of IFI44 in scallops and it may be related to the higher resistance to Vibrio bacterial in hybrids. Copyright © 2023 Elsevier Ltd. All rights reserved.

Citation

Xiaoxu Qu, Xia Wang, Bo Liu, Min Chen, Junhao Ning, Haijun Liu, Guilong Liu, Xin Xu, Xiaotong Zhang, Kai Yu, He Xu, Xia Lu, Chunde Wang. Potential roles of IFI44 genes in high resistance to Vibrio in hybrids of Argopecten scallops. Fish & shellfish immunology. 2023 Apr;135:108702