Correlation Engine 2.0
Clear Search sequence regions

Sizes of these terms reflect their relevance to your search.

The effect of substitution of L and D amino acids in amatoxin analogues is discussed in this paper. The structure of the analog where D-alanine substitutes for glycine in position 7 has been worked out in solution by two-dimensional NMR methods using a 500 MHz instrument. The combined use of COSY and NOESY two-dimensional spectra allows a clear assignment of the resonances. The use of the coupling constants permits the calculation of the phi angles of the backbone. The NOE effects reveal the through-space contacts between protons of different peptide units, thus determining the rigidity of the amatoxin structure. On these grounds it has been possible to elucidate the conformation of the amatoxin analogue that resembles very closely that of beta-amanitin, thus explaining the high inhibitory activity toward RNA polymerase B.


G Zanotti, G D'Auria, L Paolillo, E Trivellone. Synthetic amatoxin analogue. A two-dimensional proton NMR study of S-deoxy-(Ile3)-(D-Ala7)-amaninamide. Biochimica et biophysica acta. 1986 Apr 22;870(3):454-62

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 3697361

View Full Text