Correlation Engine 2.0
Clear Search sequence regions

  • eS21 (2)
  • factor (2)
  • Nob1 (1)
  • nucleic acid (1)
  • pre rrna (2)
  • ribosome (1)
  • rna (3)
  • rna precursors (2)
  • rpS2 (1)
  • rpS21 (2)
  • uS2 (2)
  • yeast (4)
  • Sizes of these terms reflect their relevance to your search.

    RpS0/uS2, rpS2/uS5, and rpS21/eS21 form a cluster of ribosomal proteins (S0-cluster) at the head-body junction near the central pseudoknot of eukaryotic small ribosomal subunits (SSU). Previous work in yeast indicated that S0-cluster assembly is required for the stabilisation and maturation of SSU precursors at specific post-nucleolar stages. Here, we analysed the role of S0-cluster formation for rRNA folding. Structures of SSU precursors isolated from yeast S0-cluster expression mutants or control strains were analysed by cryogenic electron microscopy. The obtained resolution was sufficient to detect individual 2'-O-methyl RNA modifications using an unbiased scoring approach. The data show how S0-cluster formation enables the initial recruitment of the pre-rRNA processing factor Nob1 in yeast. Furthermore, they reveal hierarchical effects on the pre-rRNA folding pathway, including the final maturation of the central pseudoknot. Based on these structural insights we discuss how formation of the S0-cluster determines at this early cytoplasmic assembly checkpoint if SSU precursors further mature or are degraded. Copyright: © 2023 Pöll et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


    Gisela Pöll, Joachim Griesenbeck, Herbert Tschochner, Philipp Milkereit. Impact of the yeast S0/uS2-cluster ribosomal protein rpS21/eS21 on rRNA folding and the architecture of small ribosomal subunit precursors. PloS one. 2023;18(3):e0283698

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 36996028

    View Full Text