Enantioseparation and mechanism study on baclofen by capillary electrophoresis and molecular modeling.

Lixia Chen, Xiangjun Li, Tingting Tian, Sen Yang, Huiqun Wang

Journal of pharmaceutical and biomedical analysis 2023 May 30

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Enantioselective analysis of chiral drugs plays a significant role in chemistry, biology and pharmacology. Baclofen, an antispasmodic chiral drug, has been widely studied due to the obvious differences in toxicity and medical activity between enantiomers. Herein, a simple and efficient method for separation of baclofen enantiomers by capillary electrophoresis was established without complicated sample derivatization and expensive instruments. Then, the molecular modeling and density functional theory were used to simulate and investigate the chiral resolution mechanism of electrophoresis, the calculated intermolecular forces were directly presented by visualization softwares. Moreover, the theoretical and experimental electronic circular dichroism (ECD) spectra of ionized baclofen were compared, and the configuration of dominant enantiomer in the nonracemic mixture can be determined by ECD signal intensity, which was proportional to the electrophoresis peak area difference of the corresponding enantiomer excess experiments. In this way, the peak order identification and configuration quantification of baclofen enantiomers in electrophoretic separation were successfully achieved without relying on a single standard. Copyright © 2023. Published by Elsevier B.V.

Citation

Lixia Chen, Xiangjun Li, Tingting Tian, Sen Yang, Huiqun Wang. Enantioseparation and mechanism study on baclofen by capillary electrophoresis and molecular modeling. Journal of pharmaceutical and biomedical analysis. 2023 May 30;229:115371