Development of a new HISCL automated CXCL9 immunoassay.

Scientific reports 2023 Apr 01

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C-X-C motif chemokine ligand 9 (CXCL9), a candidate biomarker, reflects type 1 (T1) inflammation pathology. Here, we report the analytical performance and clinical characteristics of a new CXCL9 reagent for a fully automated immunoassay device. We evaluated the limits of blank, detection, and quantitation (LoQ) along with other efficacy parameters, and the ability of the assay to report patient health, COVID-19 status, and the presence of asthma and/or interstitial lung diseases (ILDs). The coefficient of variation for 5-day total precision using two instruments was 7% across two controls, serum, and plasma panels. LoQ of 2.2 pg/mL suggested the efficacy of the assay in detecting T1 inflammation in plasma or serum; no cross-reactivity or interference was observed. We identified high serum CXCL9 levels in samples from patients with acute COVID-19 infections (n = 57), chronic bird-related hypersensitivity pneumonitis (n = 61), asthma (n = 194), and ILDs (n = 84) compared to healthy individuals (< 39.0 pg/mL). Furthermore, CXCL9 levels increased with age in asthma patients, and an opposite trend was observed for T2 inflammatory factors. These results suggest the utility of the automated CXCL9 immunoassay for measuring CXCL9 in clinical samples and reflect its role in T1 inflammation. © 2023. The Author(s).

Citation

Takehiro Hasegawa, Maho Yoshida, Shunsuke Watanabe, Takami Kondo, Hideo Asada, Atsushi Nakagawa, Keisuke Tomii, Masami Kameda, Mitsuo Otsuka, Koji Kuronuma, Hirofumi Chiba, Shinji Katayanagi, Yasunari Miyazaki, Akio Mori. Development of a new HISCL automated CXCL9 immunoassay. Scientific reports. 2023 Apr 01;13(1):5342