Imaging the T-cell receptor: new approaches, new insights.

Adam M Rochussen, Anna H Lippert, Gillian M Griffiths

Current opinion in immunology 2023 Jun

filter terms:

T cells recognize pathogenic antigens via the T-cell antigen receptor (TCR). This protein complex binds to antigen fragments on the surface of antigen-presenting cells. To understand how cellular activation can ensue rapidly from molecular recognition, the localization and distribution of the TCR on the surface of the resting T cell are of particular importance. Conflicting results regarding TCR distribution have emerged from recent studies using a range of imaging techniques, including total internal reflection and single-molecule localization microscopy modalities. Here, we review the differing results and the potential biases inherent in differing imaging approaches. In addition, we review studies showing the impact of differing imaging surfaces on T-cell activation. Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Citation

Adam M Rochussen, Anna H Lippert, Gillian M Griffiths. Imaging the T-cell receptor: new approaches, new insights. Current opinion in immunology. 2023 Jun;82:102309