Effect of Fenton oxidized lignin support on immobilized β-glucosidase activity.

Zekang Wang, Haiyan Chen, Yuyue Qin, Tianqing Lan

Journal of biotechnology 2023 May 20

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In this study, the Fenton oxidized lignin was prepared to investigate the effect of Fenton oxidation modification on the activity of lignin immobilized β-glucosidase (β-GL). The results demonstrated that Fenton oxidation could significantly improve the activity and stability of immobilized β-GL. This is because the Fenton oxidation increased the electrostatic, hydrogen bonding, and hydrophobic forces between lignin and β-GL, resulting in increased lignin adsorption onto β-GL. The Fenton oxidation also changed the chemical structure of lignin, altering the lignin-β-GL binding site and reducing the negative effect of lignin on the β-GL catalytic domain. This research will improve understanding of the effect of Fenton lignin oxidation on immobilized β-GL activity and expand the use of lignin in enzyme immobilization. Copyright © 2023 Elsevier B.V. All rights reserved.

Citation

Zekang Wang, Haiyan Chen, Yuyue Qin, Tianqing Lan. Effect of Fenton oxidized lignin support on immobilized β-glucosidase activity. Journal of biotechnology. 2023 May 20;368:31-41